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KMID : 0604520190450020175
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Journal of the Society of Cosmetic Scientists of Korea
2019 Volume.45 No. 2 p.175 ~ p.184
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Development of Dermal Transduction Epidermal Growth Factor (EGF) Using A Skin Penetrating Functional Peptide
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Kang Jin-Sun
La Ha-Na
Bak Sun-Uk
Eom Hyo-Jung
Lee Byung-Kyu
Shin Hee-Je
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Abstract
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The epidermal growth factor (EGF) has a intrinsic function of inducing growth and proliferation of cells through interacting with cell membrane receptors in human epidermis and dermis layer. These functions of EGF are used as a main ingredient for wound healing medicines and anti-aging cosmetics. As a cosmetic ingredient, the EGF has a problem in exhibiting its natural efficacy due to the lack of the ability to penetrate through the stratum corneum, which is known as the skin barrier. In this study, a recombinant human epidermal growth factor (MTD151-EGF) fused with the macromolecule transduction domain (MTD)151 with the skin penetration ability was developed to improve the skin penetration efficiency of the EGF. Expression of MTD151-EGF was performed in E. coli transformed with a vector encoding the MTD151-EGF gene and then purified. The purified MTD151-EGF was evaluated using cell proliferation assay, cytotoxicity test and skin penetration test by franz diffusion cell assay and artificial skin. Cell proliferation activity of MTD151-EGF purified to high purity of 99% or above was equivalent to the EGF or better, and cytotoxicity was not observed. In addition, the MTD151-EGF showed an excellent penetration efficiency compared to the EGF in the skin penetration test with EGF and MTD151-EGF labeled by FITC in an artificial skin penetration model. Based on the quantitative analysis of the penetrating substance using franz diffusion cell assay, the amount of penetration was about 16 times more than that of EGF. These results can be regarded as an effective alternative to improve the existing physical transdermal penetration method related to the use of various active ingredients for cosmetics.
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KEYWORD
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epidermal growth factor, MTD-EGF, recombinant human EGF, macromolecule transduction domain, MTD
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